Search

Support Feedback

Neisseria gonorrhoeae and Chlamydia trachomatis by PCR, Genital

Methodology:

Qualitative Real-Time PCR

Performed:

Mon-Fri

Analytical Time:

Within 7 days of receipt

Testing Laboratory:

Pathology Laboratory

Laboratory Section:

Microbiology

Interpretive Data:

This assay should not be used to assess therapeutic success or failure for persons treated with the CDC recommended or alternative regimens, since nucleic acids from these organisms may persist for 3 weeks or more following antimicrobial therapy. If a test-of-cure is requested, a Chlamydia culture (EPIC LAB 2166), should be requested. This test is appropriate for retesting at three months or longer post antimicrobial therapy.

THIS TEST IS FOR GENITAL SPECIMENS ONLY. If testing is needed on urine specimens, order test code UGCCT.

Neisseria gonorrhoeae and Chlamydia trachomatis by PCR, Genital

Preferred Collection Container:

Roche Cobas PCR Media

Also Acceptable Collection Container:

Universal Viral Transport Media
Aptima swab
Copan ESwab

Collection Information:

Endocervical Swab Specimen: using the cobas® PCR Female Swab Sample Packet containing PCR transport medium and swabs:
1. Remove one swab from the packaging.
2. Using the swab, remove excess mucus from the cervical os. Discard the used swab.
3. Remove second swab from the packaging.
4. Insert the second swab into the cervical canal and rotate for 15-30 seconds.
5. Withdraw the swab carefully. Avoid contact with the vaginal mucosa.
6. Immediately place the swab into the transport medium. Break the shaft of the swab, leaving the remaining swab in the transport medium. Make sure the cap is tightly secured to the tube.

Male Urethral Specimen (Note: Urine is the preferred sample for males): See Neisseria gonorrhoeae and Chlamydia Trachomatis, Urine, by PCR or using the cobas® PCR Female Swab Sample Packet containing PCR transport medium and swabs:
1. Insert the collection swab 2-4 cm into the urethra and rotate for 3-5 seconds.
2. Withdraw the swab. Immediately place the swab into the transport medium. Break the shaft of the swab, leaving the remaining swab in the transport medium. Make sure the cap is tightly secured to the tube. Discard second swab.

Transport Information:

Cobas PCR media, VTM, Aptima swabs, and ESwabs can all be transported ambient or refrigerated.

Minimum Sample Volume: Minimum amount of analytical sample (serum, plasma, urine, CSF, etc.) required to perform an assay once. Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory or reflex testing. In some situations, a minimum specimen volume may result in a QNS (Quantity Not Sufficient) result, requiring a second specimen to be collected.

Minimum amount of analytical sample (serum, plasma, urine, CSF, etc.) required to perform an assay once. Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory or reflex testing. In some situations, a minimum specimen volume may result in a QNS (Quantity Not Sufficient) result, requiring a second specimen to be collected.

1.5 mL

Remarks:

Gross presence of blood may interfere with test performance.
Collection using carbomer-containing products may generate invalid or false negative results.
STI Sample Collection Guidance - Carbomer-free and Carbomer-containing product list (2023)

Unacceptable Conditions:

Specimens NOT collected and placed in Universal viral transport medium, Aptima media, cobas® PCR media or ESwab. Wooden swabs are not acceptable.

Stability

Refrigerated Stability:

Universal Viral Transport Media: 7 days
Copan Eswab: 7 days
cobas PCR Media: 12 months
Aptima Swab: 7 days

Frozen Stability:

Universal Viral Transport Media: 30 days
Copan Eswab: 30 days

Ambient Stability:

cobas PCR Media: 12 months

Neisseria gonorrhoeae and Chlamydia trachomatis by PCR, Genital

Sunquest Order Code:

CXGCCT

Epic Number: The test code used to identify this assay in Epic

LAB2200

CPT Code:

87494

Neisseria gonorrhoeae and Chlamydia trachomatis by PCR, Genital

Modified:

1/31/2012 11:59:05 AM