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Alzheimer's Disease Evaluation, Spinal Fluid

Alias:

Amyloid Beta 42; Beta-Amyloid 42; ABeta; Total Tau; Phosphorylated Tau; pTau; tTau

Methodology:

Electrochemiluminescent Immunoassay (ECLIA)

Performed:

Tuesday, Friday

Analytical Time:

1 to 4 days

Testing Laboratory:

Mayo Medical Laboratories; Rochester, MN

Laboratory Section:

Referral Testing

Alzheimer's Disease Evaluation, Spinal Fluid

Container/Tube Type:

CSF AD Biomarker Tube (BlueTop SARSTEDT); specimens not collected in this tube will be rejected

Collection Information:

IMPORTANT: Must get CSF AD Biomarker Tube (BlueTop SARSTEDT) from Laboratory before procedure.

1. Perform lumbar puncture and discard the first 1 to 2 mL of cerebrospinal fluid (CSF).
2. Collect CSF directly into one of the listed collection tubes until the tube is at least 50% full.*

Note: Polystyrene collection tubes are not acceptable. Exposure of CSF to polystyrene tubes may result in falsely low Abeta42 concentrations.

*The Alzheimer's Association consensus protocol for handling of CSF for clinical measurements of Abeta42 and tau recommends using the drip method for CSF collection and directly collecting into a low bind polypropylene tube. Although some clinicians prefer the syringe pull method due to speed of collection, the drip method reduces the risk of Abeta42 binding to the plastic of any syringe used.

Transport Information:

Send CSF in original CSF AD Biomarker Tube (BlueTop SARSTEDT) tube, do NOT aliquot into any other container.

Minimum Sample Volume: Minimum amount of analytical sample (serum, plasma, urine, CSF, etc.) required to perform an assay once. Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory or reflex testing. In some situations, a minimum specimen volume may result in a QNS (Quantity Not Sufficient) result, requiring a second specimen to be collected.

Minimum amount of analytical sample (serum, plasma, urine, CSF, etc.) required to perform an assay once. Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory or reflex testing. In some situations, a minimum specimen volume may result in a QNS (Quantity Not Sufficient) result, requiring a second specimen to be collected.

1.5 mL to 2.5 mL of CSF in original CSF AD Biomarker Tube (BlueTop SARSTEDT) tube

Remarks:

For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Unacceptable Conditions:

CSF not collected in CSF AD Biomarker Tube, gross hemolysis, gross icterus

Stability Comments:

Stability applies only to CSF collected in the CSF AD Biomarker Tube (Blue Top SARSTEDT)

Stability

Refrigerated Stability:

14 days - Preferred

Frozen Stability:

60 days

Ambient Stability:

12 hours

Alzheimer's Disease Evaluation, Spinal Fluid

Sunquest Order Code:

ADEVLM

Epic Number: The test code used to identify this assay in Epic

LAB3736

Reference Lab Code: The test code used to identify this assay at the referral laboratory that performs this testing.

ADEVL

CPT Code:

82234; 84393; 84394

Alzheimer's Disease Evaluation, Spinal Fluid

Modified:

2/28/2014 3:37:25 PM

2/28/2014 15:37:25 PM