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Double Stranded DNA (dsDNA) Antibody IgG by Multiplex Flow Immunoassay (MFI) with reflex to IFA using Crithidia

Alias:

dsDNA Ab with Reflex IgG

Methodology:

Multiplex Flow Immunoassay (MFI)
Indirect immunofluorescence (IFA) using Crithidia luciliae

Performed:

MFI: Monday - Friday
IFA: Monday and Thursday

Analytical Time:

4 hours

Testing Laboratory:

Pathology Laboratory

Laboratory Section:

Core Laboratory

Double Stranded DNA (dsDNA) Antibody IgG by Multiplex Flow Immunoassay (MFI) with reflex to IFA using Crithidia

Preferred Collection Container:

SST (Serum Separator)

Also Acceptable Collection Container:

Red (No Additive)

Collection Information:

Collect blood in a serum separator or red top tube.

Transport Information:

Centrifuge and aliquot 0.25 mL into a plastic transport vial. Send refrigerated.

Minimum Sample Volume: Minimum amount of analytical sample (serum, plasma, urine, CSF, etc.) required to perform an assay once. Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory or reflex testing. In some situations, a minimum specimen volume may result in a QNS (Quantity Not Sufficient) result, requiring a second specimen to be collected.

Minimum amount of analytical sample (serum, plasma, urine, CSF, etc.) required to perform an assay once. Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory or reflex testing. In some situations, a minimum specimen volume may result in a QNS (Quantity Not Sufficient) result, requiring a second specimen to be collected.

0.15 mL of serum (approximately 0.3 mL of whole blood based upon a normal patient hematocrit).

Unacceptable Conditions:

Hemolysis

Stability

Refrigerated Stability:

7 days - Preferred

Frozen Stability:

30 days

Ambient Stability:

4 hours

Double Stranded DNA (dsDNA) Antibody IgG by Multiplex Flow Immunoassay (MFI) with reflex to IFA using Crithidia

Sunquest Order Code:

DSDNWR

Epic Number: The test code used to identify this assay in Epic

LAB5257

CPT Code:

86225; if reflex negative, add 86255, if reflex positive, add 86256

Double Stranded DNA (dsDNA) Antibody IgG by Multiplex Flow Immunoassay (MFI) with reflex to IFA using Crithidia

Modified:

2/22/2012 3:39:15 PM