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Antinuclear Antibodies (ANA) by Immunofluorescence with Pathologist Interpretation and Reflex to ANA10 Battery

Alias:

ANA (antinuclear antibodies); ANA; ANA1; ANA2; Antinuclear antibodies (ANA); HEp-2 substrate, serum; Antinuclear Antibody, Serum; FANA (fluorescent antinuclear antibodies); Hep-2; LE (lupus erythematosus) prep; ANA WR

Methodology:

Immunofluorescence and Multiplex Flow Immunoassay

Performed:

Monday through Friday

Analytical Time:

1 to 3 days

Testing Laboratory:

Pathology Laboratory

Laboratory Section:

Core Laboratory

Antinuclear Antibodies (ANA) by Immunofluorescence with Pathologist Interpretation and Reflex to ANA10 Battery

Preferred Collection Container:

SST (Serum Separator)

Also Acceptable Collection Container:

Red (No Additive)

Collection Information:

Collect blood in a serum separator tube. (Serum red top also acceptable).

Transport Information:

Allow blood to clot at room temperature. Centrifuge and aliquot 1.5 mL of serum to a plastic transport vial. Send refrigerated.

Minimum Sample Volume: Minimum amount of analytical sample (serum, plasma, urine, CSF, etc.) required to perform an assay once. Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory or reflex testing. In some situations, a minimum specimen volume may result in a QNS (Quantity Not Sufficient) result, requiring a second specimen to be collected.

Minimum amount of analytical sample (serum, plasma, urine, CSF, etc.) required to perform an assay once. Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory or reflex testing. In some situations, a minimum specimen volume may result in a QNS (Quantity Not Sufficient) result, requiring a second specimen to be collected.

1.0 mL of serum (approximately 2.0 mL of whole blood based upon a normal patient hematocrit)

Remarks:

ANA samples are screened at a titer of 1:80. All samples that are positive are titered and the titer and pattern will be reported. All positives include a Pathologist's interpretation.

Unacceptable Conditions:

Plasma, gross hemolysis, icterus, lipemia, bacterial growth.

Stability

Refrigerated Stability:

7 days

Frozen Stability:

Indefinitely

Antinuclear Antibodies (ANA) by Immunofluorescence with Pathologist Interpretation and Reflex to ANA10 Battery

Sunquest Order Code:

ANAWR

Epic Number: The test code used to identify this assay in Epic

LAB5463

CPT Code:

86038 if negative; If positive 86038x2 , 86039, 86235 x10, 83520

Antinuclear Antibodies (ANA) by Immunofluorescence with Pathologist Interpretation and Reflex to ANA10 Battery

Modified:

10/19/2015 6:34:03 AM