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HLA-B27

Methodology:

RT-PCR

Performed:

Monday - Friday

Analytical Time:

3 - 7 days

Testing Laboratory:

Iowa Methodist Medical Center

Laboratory Section:

Histocompatibility Laboratory (HLA)

HLA-B27

Preferred Collection Container:

Lavender (EDTA)

Also Acceptable Collection Container:

Yellow (ACD-A or ACD-B)
Pink (K2EDTA)

Collection Information:

Collect blood in a lavender (EDTA) tube. Yellow ACD A or B and Pink (EDTA) is also acceptable. This test requires it's own collection tube (sharing between departments is NOT acceptable).

Transport Information:

Transport original tube at room temperature. A separate EDTA (lavender-top) tube must be submitted for all HLA testing. HLA-B27 testing requires its own specimen separate and cannot be shared with other laboratory sections. Do not open tube.

Minimum Sample Volume: Minimum amount of analytical sample (serum, plasma, urine, CSF, etc.) required to perform an assay once. Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory or reflex testing. In some situations, a minimum specimen volume may result in a QNS (Quantity Not Sufficient) result, requiring a second specimen to be collected.

Minimum amount of analytical sample (serum, plasma, urine, CSF, etc.) required to perform an assay once. Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory or reflex testing. In some situations, a minimum specimen volume may result in a QNS (Quantity Not Sufficient) result, requiring a second specimen to be collected.

3.0 mL of whole blood

Unacceptable Conditions:

Aliquot, unlabeled or mislabeled samples will be rejected. HLA will determine specimen acceptability for reasons other than unlabeled/mislabeled.

Stability Comments:

All specimens will be evaluated by the Histocompatibility Department for test suitability.

Stability

Refrigerated Stability:

4 days

Frozen Stability:

NOT Acceptable

Ambient Stability:

4 days - Preferred

HLA-B27

Sunquest Order Code:

B27HLA

Epic Number: The test code used to identify this assay in Epic

LAB4946

CPT Code:

81374

HLA-B27

Modified:

2/23/2012 12:56:12 PM