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Syphilis Antibody, IgG and IgM

Alias:

FTA-ABS, TPPA, Treponema pallidum, Syphilis screen, FTA, VDRL

Methodology:

Multiplex Flow Immunoassay

Performed:

Daily

Analytical Time:

1 day

Testing Laboratory:

Pathology Laboratory

Laboratory Section:

Core Laboratory

Interpretive Data:

If equivocal or reactive, this test will automatically reflex to the RPR confirmation (RPRCNF) assay.
If the RPR Confirmation is reactive, it will be titered.
If the RPR Confirmation is nonreactive, an order for the Syphilis Antibody, Treponema pallidum Particle Agglutination, Serum (TPPA) will be ordered reflexively.
Testing results are reported to Iowa Health and Human Services (formerly Iowa Department of Health)
Syphilis Screening vs. Monitoring Algorithm

Syphilis Antibody, IgG and IgM

Preferred Collection Container:

SST (Serum Separator)
Light Green (Lithium Heparin Plasma Separator Tube)

Also Acceptable Collection Container:

Red (No Additive)
Green No Gel (Lithium Heparin)
EDTA plasma specimens are acceptable but will not route in Sunquest.

Collection Information:

Collect in a SST (Serum Separator) or Light Green (Lithium Heparin Plasma Separator Tube). Red (No Additive), Green No Gel (Lithium Heparin), and Lavender (EDTA) also acceptable.

Transport Information:

Centrifuge and aliquot 0.5 mL of serum or plasma into a plastic transport vial. Send refrigerated.

Minimum Sample Volume: Minimum amount of analytical sample (serum, plasma, urine, CSF, etc.) required to perform an assay once. Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory or reflex testing. In some situations, a minimum specimen volume may result in a QNS (Quantity Not Sufficient) result, requiring a second specimen to be collected.

Minimum amount of analytical sample (serum, plasma, urine, CSF, etc.) required to perform an assay once. Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory or reflex testing. In some situations, a minimum specimen volume may result in a QNS (Quantity Not Sufficient) result, requiring a second specimen to be collected.

0.25 mL of serum (approximately 0.5 mL of blood based upon a normal patient's hematocrit)

Unacceptable Conditions:

Grossly hemolyzed or lipemic specimens.

Stability

Refrigerated Stability:

7 days - Preferred

Frozen Stability:

Indefinitely

Syphilis Antibody, IgG and IgM

Sunquest Order Code:

SYPHGM

Epic Number: The test code used to identify this assay in Epic

LAB5650

CPT Code:

86780

Syphilis Antibody, IgG and IgM

Modified:

10/19/2015 6:34:03 AM