Syphilis Antibody, IgG and IgM
Alias:
FTA-ABS, TPPA,
Treponema pallidum, Syphilis screen, FTA, VDRL
Methodology:
Multiplex Flow Immunoassay
Performed:
Analytical Time:
Testing Laboratory:
Laboratory Section:
Interpretive Data:
If positive, this test will automatically reflex to the RPR confirmation (RPRCNF) assay.
If the RPR is positive, it will be titered.
If the RPR is negative, an order for the Syphilis Antibody, Treponema pallidum Particle Agglutination, Serum (TPPA) will be ordered reflexively.
All positive results are reported to the State of Iowa Department of Public Health.
Syphilis Screening vs. Monitoring Algorithm
|
STYPPA
Result
|
Confirmatory
Result (RPR)
|
UHL - TPPA
Result
|
Interpretation
|
|
Nonreactive
|
N/A
|
N/A
|
No serologic evidence of syphilis. No follow-up necessary unless clinically indicated.
|
|
Equivocal
|
N/A
|
N/A
|
These results indicate the possibility of untreated or recently treated syphilis, but most likely represent a false reactivity. Repeat testing in 3-4 weeks if clinically indicated.
|
|
Reactive*
|
Reactive*
|
N/A
|
These results represent untreated or recently treated syphilis.
|
|
Reactive*
|
Nonreactive
|
Reactive*
|
Possible syphilis (early or latent) or previously treated syphilis. Historical and clinical evaluation required.
|
|
Reactive*
|
Nonreactive
|
Nonreactive
|
Probable false-reactive syphilis STYPPA test. No follow up testing unless clinically indicated.
|
*Reactive results are reported to the Iowa Department of Public Health.
Syphilis Antibody, IgG and IgM
Container/Tube Type:
Red (No Additive)
SST (Serum Separator)
Also Acceptable Collection Container:
EDTA plasma specimens are acceptable but will not route in Sunquest.
Collection Information:
Collect 1.0 mL of blood in red top or serum separator tube.
Transport Information:
Send 0.5 mL of serum refrigerated in a transport vial.
Minimum Sample Volume: 
0.25 mL of serum (approximately 0.5 mL of blood based upon a normal patient's hematocrit)
Remarks:
Unacceptable Conditions:
Grossly hemolyzed or lipemic specimens.
Stability
Refrigerated Stability:
Frozen Stability:
Syphilis Antibody, IgG and IgM
Sunquest Order Code:
Epic Number: CPT Code:
Syphilis Antibody, IgG and IgM
Modified: